Pharma-2012 : Preparation of BSA nanoparticles by desolvation technique using ethyl alcohol as desolvating agent - A.Krishna Sailaja - Osmania University

In order to see functionality and toxicity of nanoparticles in various food and drug applications, it is important to establish procedures to prepare nanoparticles of a controlled size. Desolvation, a thermodynamically driven self-assembly process for polymeric materials.Here BSA nanoparticles were prepared by desolvation technique using Ethyl alcohol as desolvating agent. In our study, Ethyl alcohol was added intermittently into 1% BSA solution under stirring at 700 rpm. Amount of Ethanol added, intermittent timeline of Ethanol addition, and pH of solution were considered as process parameters to be optimized. Effect of the process parameters on size of the nanoparticles were studied. The results indicated that the size control of BSA nanoparticle was achieved by adding Ethanol intermittently. The standard deviation of average size of BSA nanoparticles at each preparation condition was minimized by adding Ethanol intermittently. Particle size of BSA nanoparticles prepared by continuous addition of Ethyl alcohol as desolvating agent was in between 200-300nm.Whereas uniform particle size was obtained by adding Ethyl alcohol intermittently. The particle size was found to be in between 180-220nm.FTIR spectra Indicates that there was no drug and polymer interaction. A nanoparticle or ultrafine particle is typically defined as a particle of matter that's between 1 and 100 nanometres in diameter. The term is usually used for larger particles, up to 500 nm, or fibers and tubes that are but 100 nm in just two directions. Nanoparticles are now bOut of three human studies, just one showed a passage of inhaled nanoparticles into the bloodstream. Materials which by themselves aren't very harmful might be toxic if they're inhaled within the sort of nanoparticles. The consequences of inhaled nanoparticles within the body may include lung inflammation and heart problems. 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Author(s):

A.Krishna Sailaja and P. Amareshwar

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